Presents:
More than 1200 scientists from around the world have strongly recommended this intensive course as an opportunity to develop protein research and analytical skills in a retreat setting. Participants work hard, identify and solve problems in the lab and enjoy comaraderie and good food and drink with colleagues.
This five and one half day laboratory course covers a wide variety of conventional methods for protein isolation, purification, and characterization. The course format integrates hands-on laboratory exercises with classroom lectures, demonstrations, study breaks, and short take-home assignments.
A special feature of the course is that all laboratory work will be performed on the same starting sample (Aequorea GFP or recombinant GFP), which will be purified from an exceedingly crude form (starting with tissue or bacterial cell extraction) to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing, and western blotting. This feature provides a continuity of purpose, integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match.
A problem-solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data, and interpret results. A student-teacher ratio not greater than 8:1 will be maintained and the faculty coordinators will be present throughout the course.
*Introduced in problem-solving workshop, Friday
Course participants will extract an easily visualized chromoprotein, the green-fluorescent protein, (Science vol. 263 pp. 802-805,1994) from a frozen tissue sample or bacterial cell pellets, clarify the extract, and then concentrate and purify the protein by "salting out". Gel filtration, ion exchange, hydrophobic interaction, and size exclusion HPLC chromatography will then be employed to extensively purify the desired protein (GFP) from the crude extract. The unique nature of this brilliantly fluorescent protein allows you to follow all phases of the purification with a simple hand-held mineral light, enhancing the students' understanding of each process.
The purified protein will be characterized by SDS and native gel electrophoresis, isoelectric focusing, ion exchange HPLC, size exclusion HPLC, and Western blotting. Each group will prepare a detailed purification table and graphs (homework assignments), and will characterize the protein with respect to purity, charge, molecular weight, isoelectric point, unique spectral features, subunit composition, isoprotein composition, and the chemical nature of the chromogenic peptide.
This course integrates lecture and laboratory sessions to provide a comprehensive learning experience. The course begins with an introductory lecture on Sunday afternoon followed by a delightful dinner at a local restaurant. Everyone is strongly encouraged to attend this session, but participants who cannot arrive for the Sunday lecture may begin the course on Monday morning (at the laboratory location).
The course concludes Friday afternoon with an interactive problem-solving workshop and a Molecular modeling workshop at the Rutgers Structural Biology Computational Laboratory facilities at Cook College, Rutgers.
Dr. William Ward
Associate Professor of Biochemistry, Cook College, Rutgers
University and Director of C.R.E.B.B. Dr. Ward is a noted teacher and seminar
leader with more than 20 years of experience with adult professional audiences.
He specializes in the chemical and physical properties of the green-fluorescent
protein and other proteins involved in marine bioluminescence. Dr. Ward also
teaches "Fluorescence: Basic Principles and Applications in Drug Discovery"
for the IBC Life Sciences Training Academy.
Dr. Ward has just co-authored (with Catherine Thomson,
Ph.D.) " A Guide to Green-Fluorescent Protein: Applications in Cell Biology
and Drug Discovery " for D&MD Publications. This resource-oriented practical
guide presents an overview of the most important features and technological
applications of Green-Fluorescent Protein (GFP) and its variant forms, and illustrates
how GFP is currently being used in the biopharmaceutical industry.
D&MD Publishing is happy to extend a 10% discount to the registrants of the
Protein Purification course and Biochemical Separations course. When ordering
this Guide, please mention source code "9133-10%PP". To order the Guide, please
contact D&MD's Customer Service Department at cust.serv@drugandmarket.com or call (508)616-5566.
Dr. Gavin Swiatek, Post
Doctoral Associate, Microbiology, Cook College, Rutgers University
Ms. Caren Villano, Graduate Assistant,
Biochemistry, Cook College, Rutgers University
Dr. Richard Ludescher, Professor of Food
Science, Cook College, Rutgers University
Mr. Robert Muldowney, Associate Director,
Structural Biology Computational Laboratory, Cook College, Rutgers University
What Your Colleagues Say About "Protein Purification" at Rutgers
“I was extremely impressed
with the quality of the course and caliber of all instructors ... best course
like this I’ve been to ...
Thank you for a wonderful learning opportunity and environment ...”
Margo Zanotto, Manufacturing Associate, Biotech, Baxter Biotech
"Bill gave an interesting and entertaining lecture. There were great analogies and stories specifically relating to his experiences." Cindy Ali, Product Manager, BioRad Laboratories
"Starting with the actual jellyfish and working
with the same sample was great...l enjoyed the day-to-day progress. "
Rebecca Trerise, Applications Engineer, Millipore, Inc.
"Dr. Ward was very available and open to questions.
He sincerely tried to answer every question asked and was very patient.
The TAs were awesome, very well prepared and eager to help."
Bridget Ferdinand, Manufacturing Associate, Antigenics, L.L.C.
"I got far more out of the course than I had even
anticipated, and it was certainly money VERY WELL spent. In that one week, the
training was far more intense and the knowledge I gained more directly applicable
to what I am working on than I could have attained otherwise. I still refer
to the manual that you provided. I also appreciated the assistance and knowledge
of your collaborators."
Robert A. Bollinger, Dept. of Radiology, UT Southwestern Medical Center
The Center for Research and Education in Bioluminescence and Biotechnology (C.R.E.B.B) is a component of Rutgers University, Cook College, and offers a series of continuing education workshops each year featuring nationally renowned presenters. The CREBB mission is to perform basic research on bioluminescence and to utilize bioluminescence (especially the Green Fluorescent Protein) as a tool to educate the scientific and industrial communities in the field of biotechnology.
Registration
Information
The tuition fee for this five and one-half day course is $2,695, register three
weeks prior to the start of the course and pay only $2,195.
A discounted fee of $1,995 per person is available if two or more registrants
from the same company register. There is also a
discounted fee of $1,395 for attendees from academia. Fees MUST BE PAID three
(3) weeks prior to the start of the course to be eligible
for the discounted rates. The tuition fee includes continental breakfasts, lunches,
coffee breaks and three dinners, along with all
course materials, hand-outs and an instructional CD.
All fees are discounted $200 for January
REGISTER
NOW!!
Also visit
Biochemical Separations:
Introduction to Laboratory Techniques
(2 days, lecture/demonstration) for a less rigorous two-day
treatment of concepts in protein purification
This course provides an introduction to techniques
used to isolate, purify and characterize biological
macromolecules. Emphasis is placed on techniques used in protein isolation
and purification –
both from native and recombinant sources. Techniques include extraction, filtration,
centrifugation,
column chromatography including HPLC, electrophoresis, isoelectric focusing,
western blotting and
capillary zone electrophoresis. This is an introductory level course designed
for scientists having
limited experience with laboratory separations.
May 18-19, 2006
July 6-7, 2006
Links to International Symposia organized by Dr. Ward:
Calcium
Regulated Photoproteins and
Green-Fluorescent Proteins - Symposium
For questions or requests for additional information concerning Protein Purification:
Rutgers University, Cook College
(CREBB)
Department of Biochemistry and Microbiology
76 Lipman Drive, 2nd floor
New Brunswick, NJ 08901-8525
Dr. Bill Ward
(Director) - crebb@rci.rutgers.edu
phone: 732-932-9562 ext 216
Gloria Kierniesky
(Program Coordinator) - Kierniesky@AESOP.Rutgers.edu
phone: 732-932-9562 ext 225
Directions to our facilities at Lipman Hall: http://www.cook.rutgers.edu/~dbm/Directions.html
Rutgers Information Service at (732) 932-INFO. Please visit the following Rutgers
web site for information on
discounted travel and lodging: http://www.rutravel.rutgers.edu/discountprograms.html
For convenient on-campus accomodations within easy walking distance, we recommend
the University Inn and Conference
Center
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